Bethyl Laboratories
Rabbit anti-Cyclin E1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00341
TargetCyclin E1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
Image 1 of 1
local_shipping
Order within 13 hours and 8 minutes for shipment by Tuesday, November 10
Product Description
Cyclin E1 is a regulatory subunit of the CDK2 kinase. Cyclin E is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 360 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCyclin E1
Antigen Species
Human
Gene ID898
Gene Symbol
CCNE1
Gene Name
cyclin E1
Uniprot IDP24864
Protein Name
G1/S-specific cyclin-E1
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Cyclin E1 immobilized on solid support.
The epitope recognized by IHC-00341 maps to a region between residue 360 and 410 of human cyclin E1 using the numbering given in entry NP_001229.1 (GeneID 898).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00341Open
Product DatasheetPDF · IHC-00341 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00341 · Lot 1 (current lot)Open
Citations / Publications
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1