Bethyl Laboratories
Rabbit anti-Cyclin L2 Antibody Affinity Purified
Primary
Catalog #A301-677A
TargetCyclin L2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Cyclin L2 is a member of the cyclin family of regulatory proteins that associate with and activate cyclin-dependent kinases (CDKs) to regulate cell cycle progression and transcription. The hallmark characteristic of cyclins is the periodicity of their expression and activity that is regulated transcriptionally and post-transcriptionally via phosphorylation and ubiquitination. Cyclin L2 is part of a group of cyclins that are involved in transcriptional regulation at the level of RNA transcription, initiation, elongation, and RNA processing. The transcriptional regulating cyclins include cyclin C, H, K, L1, L2, T1, and T2 which associate with CDK7, -8, -9, -10 and -12. Cyclin L2 associates with CDK11 and RNA polymerase II and co-localizes with splicing factors and is implicated to play a role in pre-mRNA splicing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 470 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCyclin L2
Antigen Species
Human
Gene ID81669
Gene Symbol
CCNL2
Gene Name
cyclin L2
Uniprot IDQ96S94
Protein Name
Cyclin-L2
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Cyclin L2 immobilized on solid support.
The epitope recognized by A301-677A maps to a region between residue 470 and 520 of human cyclin L2 using the numbering given in entry NP_112199.2 (GeneID 81669).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A301-677A · Lot 3 (current lot)Open
Product DatasheetPDF · A301-677A · Lot 3 (current lot)Open
Safety Data SheetPDF · A301-677AOpen
Citations / Publications
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