Rabbit anti-CYFIP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-996A maps to a region between residue 1203 and 1253 of human Cytoplasmic FMR1 Interacting Protein 1 using the numbering given in entry NP_055423.1 (GeneID 23191).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CYFIP1 immobilized on solid support.
The epitope recognized by A302-996A-T maps to a region between residue 1203 and 1253 of human Cytoplasmic FMR1 Interacting Protein 1 using the numbering given in entry NP_055423.1 (GeneID 23191).
Additional Product Information
Cytoplasmic FMR1-interacting protein 1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. CYFIP1 promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA. CYFIP1 is involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. CYFIP1 may act as an invasion suppressor in cancers [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q7L576].
Alternate Names
cytoplasmic FMR1-interacting protein 1; cytoplasmic FMRP interacting protein 1; P140SRA-1; selective hybridizing clone; SHYC; specifically Rac1-associated protein 1; SRA1; SRA-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.