Rabbit anti-Cytokeratin 8 Recombinant Monoclonal Antibody [BLR299M]
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 433 and the C-terminus (residue 483) of human Cytoskeletal keratin 8, type II using the numbering given in entry NP_002264.1 (Gene ID 3856).
Additional Product Information
Cytokeratin 8 is a member of the type II keratin family. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cytokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells where it plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. [taken from NCBI Entrez Gene (Gene ID: 3856)].
Alternate Names
CARD2;CK8;CK-8;CYK8;cytokeratin-8;K2C8;K8;keratin 8, type II;keratin, type II cytoskeletal 8;Keratin-8;KO;type-II keratin Kb8
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.