Bethyl Laboratories
Rabbit anti-DAP5 Antibody Affinity Purified
Primary
Catalog #A302-248A
TargetDAP5
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
DAP5 (death associated protein 5) is also known as eukaryotic translation initiation factor 4 gamma 2. DAP5 has been found to function as a general repressor of translation and appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis, and viral infection.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDAP5
Antigen Species
Human
Gene ID1982
Gene Symbol
EIF4G2
Gene Name
eukaryotic translation initiation factor 4 gamma 2
Uniprot IDP78344
Protein Name
Eukaryotic translation initiation factor 4 gamma 2
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DAP5 immobilized on solid support.
The epitope recognized by A302-248A maps to a region between residue 125 and 175 of human death-associated protein 5 (eukaryotic translation initiation factor 4 gamma, 2) using the numbering given in entry NP_001409.1 (GeneID 1982).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-248A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-248A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-248AOpen
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