Bethyl Laboratories

Rabbit anti-DAP5 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00673
TargetDAP5
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
Detection of human DAP5 by immunohistochemistry.
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Product Description

DAP5 (death associated protein 5) is also known as eukaryotic translation initiation factor 4 gamma 2. DAP5 has been found to function as a general repressor of translation and appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis, and viral infection.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetDAP5
Antigen Species
Human
Gene ID1982
Gene Symbol
EIF4G2
Gene Name
eukaryotic translation initiation factor 4 gamma 2
Uniprot IDP78344
Protein Name
Eukaryotic translation initiation factor 4 gamma 2
Alternate Names
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to DAP5 immobilized on solid support.

The epitope recognized by IHC-00673 maps to a region between residue 125 and 175 of human death-associated protein 5 (eukaryotic translation initiation factor 4 gamma, 2) using the numbering given in entry NP_001409.1 (GeneID 1982).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500

Documents

Safety Data SheetPDF · IHC-00673Open
Product DatasheetPDF · IHC-00673 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00673 · Lot 1 (current lot)Open
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