Bethyl Laboratories
Rabbit anti-DC8 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00478
TargetDC8
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
DC8 is a component of the MIS12 complex which includes DC8, MIS12, DSN1, and PMF1. The MIS12 complex localizes at inner kinetochores to perform an essential function in kinetochore assembly which allows proper chromosome alignment and cell cycle progression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetDC8
Antigen Species
Human
Gene ID25936
Gene Symbol
NSL1
Gene Name
NSL1, MIS12 kinetochore complex component
Uniprot IDQ96IY1
Protein Name
Kinetochore-associated protein NSL1 homolog
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DC8 immobilized on solid support.
The epitope recognized by IHC-00478 maps to a region between residue 150 and 200 of human DC8 (C1orf48) using the numbering given in entry NP_056286.2 (GeneID 25936 ).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00478Open
Product DatasheetPDF · IHC-00478 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00478 · Lot 1 (current lot)Open
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