Bethyl Laboratories

Rabbit anti-DDX1 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00132
TargetDDX1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
Detection of human DDX1 by immunohistochemistry.
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Product Description

DDX1 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX1 has been found to associate with factors involved in 3’-end cleavage and polyadenylation of pre-mRNAs.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 525 and 575
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetDDX1
Antigen Species
Human
Gene ID1653
Gene Symbol
DDX1
Gene Name
DEAD-box helicase 1
Uniprot IDQ92499
Protein Name
ATP-dependent RNA helicase DDX1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to DDX1 immobilized on solid support.

The epitope recognized by IHC-00132 maps to a region between residues 500 and 550 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 using the numbering given in entry AAH12739.1 (GeneID 1653).

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00132Open
Product DatasheetPDF · IHC-00132 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00132 · Lot 1 (current lot)Open

Citations / Publications

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