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Rabbit anti-DDX19 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
DDX19
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 400 and 450
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Bovine
Antigen Species
Human
Stock Concentration
40 µg/ml
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to DDX19 immobilized on solid support.

The epitope recognized by A300-546A-T maps to a region between residues 400 and 450 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B using the numbering given in entry NP_009173.1 (GeneID 11269). The epitope is highly conserved with DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed.

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Gene & Protein Information

DDX19 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX19 may be involved in mRNA transport.

11269

DDX19B

DEAD-box helicase 19B

Q9UMR2

ATP-dependent RNA helicase DDX19B

Alternate Names

ATP-dependent RNA helicase DDX19, ATP-dependent RNA helicase DDX19B, DBP5, DDX19, DEAD (Asp-Glu-Ala-As) box polypeptide 19B, DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog), DEAD-box protein 5, DEAD-box RNA helicase DEAD5, RNAh, yeast Dbp5 homolog

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.