Bethyl Laboratories
Rabbit anti-DDX21 Antibody Affinity Purified
Primary
Catalog #A300-628A
TargetDDX21
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DDX21 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. DDX21 has been shown to be required for the processing of 20S rRNA to 18S and has also been shown to be important for c-Jun transcriptional activation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetDDX21
Antigen Species
Human
Gene ID9188
Gene Symbol
DDX21
Gene Name
DExD-box helicase 21
Uniprot IDQ9NR30
Protein Name
Nucleolar RNA helicase 2
Alternate Names
DEAD (Asp-Glu-Ala-Asp) box helicase 21 • DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 • DEAD box protein 21 • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21 • DEAD-box helicase 21 • Gu protein • GUA • gu-alpha • GURDB • nucleolar RNA helicase 2 • nucleolar RNA helicase Gu • nucleolar RNA helicase II • RH II/Gu • RH-II/GU • RH-II/GuA • RNA helicase II/Gu alpha
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX21 immobilized on solid support.
The epitope recognized by A300-628A maps to a region between residues 50 and 100 of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-628A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-628A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-628AOpen
Citations / Publications
Services
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