Bethyl Laboratories
Rabbit anti-DDX24 Antibody Affinity Purified
Primary
Catalog #A300-697A
TargetDDX24
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DDX24 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The specific function of DDX24 has not been elucidated.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDDX24
Antigen Species
Human
Gene ID57062
Gene Symbol
DDX24
Gene Name
DEAD-box helicase 24
Uniprot IDQ9GZR7
Protein Name
ATP-dependent RNA helicase DDX24
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX24 immobilized on solid support.
The epitope recognized by A300-697A maps to a region between residue 325 and 375 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 using the numbering given in entry NP_065147.1 (GeneID 57062).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A300-697A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-697A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-697AOpen
Citations / Publications
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