Bethyl Laboratories
Rabbit anti-DDX51 Antibody Affinity Purified
Primary
Catalog #A301-089A
TargetDDX51
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 14 hours and 41 minutes for shipment by today
Product Description
DDX51 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX51 may function in biogenesis of the 60S ribosomal subunit.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 616 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetDDX51
Antigen Species
Human
Gene ID317781
Gene Symbol
DDX51
Gene Name
DEAD-box helicase 51
Uniprot IDQ8N8A6
Protein Name
ATP-dependent RNA helicase DDX51
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX51 immobilized on solid support.
The epitope recognized by A301-089A maps to a region between residue 616 and 666 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 51 using the numbering given in entry NP_778236.1 (GeneID 317781).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-089A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-089A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-089AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1