Bethyl Laboratories
Rabbit anti-DDX6 Antibody Affinity Purified
Primary
Catalog #A300-461A
TargetDDX6
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
DDX6 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX6 has been observed to positively regulate growth by modulating gene expression at the translational level.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 433 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDDX6
Antigen Species
Human
Gene ID1656
Gene Symbol
DDX6
Gene Name
DEAD-box helicase 6
Uniprot IDP26196
Protein Name
Probable ATP-dependent RNA helicase DDX6
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX6 immobilized on solid support.
The epitope recognized by A300-461A maps to a region between residues 425 and 483 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry NP_004388.2 (GeneID 1656).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 to 1:5000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:25,000 - 1:50,000
Documents
Product DatasheetPDF · A300-461A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-461A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-461AOpen
Citations / Publications
Services
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