Bethyl Laboratories
Rabbit anti-DEC1 Antibody Affinity Purified
Primary
Catalog #A300-649A
TargetDEC1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
DEC1 is a basic helix-loop-helix transcription factor that appears to play a role in cell differentiation, proliferation, apoptosis, and the circadian rhythm. DEC1 is able to promote chondrogenic and osteogenic differentiation in mesenchymal stem cells by activating transcription of bone-related genes. When it is overexpressed in cells it can suppress apoptosis by blocking caspase activation and inducing transcription of survivin. DEC1 has also been found to play a role mammalian in circadian rhythm as a suppressor of the CLOCK/BMAL1 promoter.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 362 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDEC1
Antigen Species
Human
Gene ID8553
Gene Symbol
BHLHE40
Gene Name
basic helix-loop-helix family member e40
Uniprot IDO14503
Protein Name
Class E basic helix-loop-helix protein 40
Alternate Names
basic helix-loop-helix domain containing, class B, 2 • BHLHB2 • bHLHe40 • class B basic helix-loop-helix protein 2 • class E basic helix-loop-helix protein 40 • Clast5 • DEC1 • differentially expressed in chondrocytes 1 • differentially expressed in chondrocytes protein 1 • differentiated embryo chondrocyte expressed gene 1 • enhancer-of-split and hairy-related protein 2 • HLHB2 • SHARP2 • SHARP-2 • stimulated by retinoic acid gene 13 protein • STRA13 • Stra14
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DEC1 immobilized on solid support.
The epitope recognized by A300-649A maps to a region between residues 350 and the C-terminus (residue 412) of human Differentially Expressed in Chondrocytes (Basic Helix-Look-Helix Domain Containing, Class B, 2) using the numbering given in entry NP_003661.1 (GeneID 8553).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 to 1:2000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-649A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-649A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-649AOpen
Citations / Publications
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