Bethyl Laboratories
Rabbit anti-DEK Antibody Affinity Purified
Primary
Catalog #A301-334A
TargetDEK
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
DEK is a chromatin-binding protein that may serve as an architectural protein at promoter or enhancer sites of particular genes and may be involved in the regulation of transcription and mRNA processing. DEK has been identified as an oncoprotein and an autoantigen.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDEK
Antigen Species
Human
Gene ID7913
Gene Symbol
DEK
Gene Name
DEK proto-oncogene
Uniprot IDP35659
Protein Name
Protein DEK
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DEK immobilized on solid support.
The epitope recognized by A301-334A maps to a region between residue 225 and 275 of human DEK oncogene using the numbering given in entry NP_003463.1 (GeneID 7913).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-334A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-334A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-334AOpen
Services
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Antibody and conjugate formats developed together, built to your specification.
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