/ ANTIBODIES/ANTISERA / Apoptosis Pathway Antibodies / Rabbit anti-DFF45/DFFA Antibody
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Apoptosis Pathway Antibodies  

Rabbit anti-DFF45/DFFA Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

DFF45/DFFA

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 281 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to DFF45/DFFA immobilized on solid support.

The epitope recognized by A301-420A maps to a region between residue 271 and 331 of human DNA fragmentation factor, 45kDa, alpha polypeptide using the numbering given in entry NP_004392.1 (GeneID 1676).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to DFF45/DFFA immobilized on solid support.

The epitope recognized by A301-420A-T maps to a region between residue 271 and 331 of human DNA fragmentation factor, 45kDa, alpha polypeptide using the numbering given in entry NP_004392.1 (GeneID 1676).

Additional Product Information

DFF (DNA fragmentation factor) is the principle endonuclease responsible for DNA cleavage during apoptosis and exists as a heterodimer formed between the inhibitory subunit, DFF45, and the DNA nuclease, DFF40. DFF35/DFFA is a 35 kDa splice variant of the inhibitory subunit, DFF45. Similar to DFF45, DFF35 has been shown to heterodimerize with DFF40 and also negatively regulate its nuclease activity. In addition to regulating DFF40 activity, DFF35 has also been found to assist in the synthesis and folding of the DFF40 endonuclease.

1676

DFFA

DNA fragmentation factor subunit alpha

O00273

DNA fragmentation factor subunit alpha

Alternate Names

DFF1; DFF45; DFF-45; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; inhibitor of CAD

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.