Bethyl Laboratories
Rabbit anti-DFF45/DFFA IHC Antibody Affinity Purified
Primary
Catalog #IHC-00291
TargetDFF45/DFFA
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
DFF (DNA fragmentation factor) is the principle endonuclease responsible for DNA cleavage during apoptosis and exists as a heterodimer formed between the inhibitory subunit, DFF45, and the DNA nuclease, DFF40. DFF35/DFFA is a 35 kDa splice variant of the inhibitory subunit, DFF45. Similar to DFF45, DFF35 has been shown to heterodimerize with DFF40 and also negatively regulate its nuclease activity. In addition to regulating DFF40 activity, DFF35 has also been found to assist in the synthesis and folding of the DFF40 endonuclease.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 281 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetDFF45/DFFA
Antigen Species
Human
Gene ID1676
Gene Symbol
DFFA
Gene Name
DNA fragmentation factor subunit alpha
Uniprot IDO00273
Protein Name
DNA fragmentation factor subunit alpha
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DFF45/DFFA immobilized on solid support.
The epitope recognized by IHC-00291 maps to a region between residue 271 and 331 of human DNA fragmentation factor, 45kDa, alpha polypeptide using the numbering given in entry NP_004392.1 (GeneID 1676).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00291Open
Product DatasheetPDF · IHC-00291 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00291 · Lot 1 (current lot)Open
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