Bethyl Laboratories
Rabbit anti-DGCR8 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00688
TargetDGCR8
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
DiGeorge syndrome critical region 8 (DGCR8) is a component of the microprocessor complex that acts as a RNA- and heme-binding protein and is involved in the initial step of microRNA (miRNA) biogenesis. DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junctions. It directs RNASEN/DROSHA to cleave 11 bp away from the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8WYQ5].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetDGCR8
Antigen Species
Human
Gene ID54487
Gene Symbol
DGCR8
Gene Name
DGCR8, microprocessor complex subunit
Uniprot IDQ8WYQ5
Protein Name
Microprocessor complex subunit DGCR8
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DGCR8 immobilized on solid support.
The epitope recognized by IHC-00688 maps to a region between residue 1 and 50 of human DiGeorge syndrome critical region gene 8 using the numbering given in entry NP_073557.3 (GeneID 54487).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00688Open
Product DatasheetPDF · IHC-00688 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00688 · Lot 1 (current lot)Open
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