Bethyl Laboratories
Rabbit anti-DGKzeta Antibody Affinity Purified
Primary
Catalog #A302-967A
TargetDGKzeta
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
Image 1 of 2
local_shipping
Order within 3 hours and 22 minutes for shipment by Tuesday, November 17
Product Description
Diacylglycerol kinase, zeta, belongs to the eukaryotic diacylglycerol kinase family, a family of enzymes that convert DAG to phosphatidic acid (PA).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 878 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDGKzeta
Antigen Species
Human
Gene ID8525
Gene Symbol
DGKZ
Gene Name
diacylglycerol kinase zeta
Uniprot IDQ13574
Protein Name
Diacylglycerol kinase zeta
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DGKzeta immobilized on solid support.
The epitope recognized by A302-967A maps to a region between residue 1067 and 1117 of human Diacylglycerol Kinase Zeta using the numbering given in entry NP_001099010.1 (GeneID 8525).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A302-967A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-967A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-967AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1