Bethyl Laboratories
Rabbit anti-DHX15 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00390
TargetDHX15
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
DHX15 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX15 is the human ortholog of the yeast Prp43 protein. DHX15 has been demonstrated to function in ribosome biogenesis and pre-mRNA splicing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 350 and 400
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
50 µg/ml
Target Identity
TargetDHX15
Antigen Species
Human
Gene ID1665
Gene Symbol
DHX15
Gene Name
DEAH-box helicase 15
Uniprot IDO43143
Protein Name
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Alternate Names
ATP-dependent RNA helicase #46 • DBP1 • DDX15 • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15 • DEAD/H box-15 • DEAH (Asp-Glu-Ala-His) box helicase 15 • DEAH (Asp-Glu-Ala-His) box polypeptide 15 • DEAH box protein 15 • HRH2 • pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 • PRP43 • PRPF43 • PrPp43p • putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 • RNA helicase 2
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DHX15 immobilized on solid support.
The epitope recognized by IHC-00390 maps to a region between residues 350 and 400 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00390Open
Product DatasheetPDF · IHC-00390 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00390 · Lot 1 (current lot)Open
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