/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-DHX9 Antibody
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Primary Antibodies  

Rabbit anti-DHX9 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (21)
Target:

DHX9

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1220 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

DHX9 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAH box proteins also exhibit functions involved in transcriptional regulation. DHX9 has been shown to act as a bridge between CREB-binding protein (CBP) and RNA polymerase II to coactivate CBP-dependent transcription. A similar observation has been reported for an interaction with BRCA1. DHX9 also exhibits activities associated with nuclear export of retroviral RNAs, translation, RNA editing, and snRNP assembly.

1660

DHX9

DExH-box helicase 9

Q08211

ATP-dependent RNA helicase A

Alternate Names

ATP-dependent RNA helicase A; DDX9; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box helicase 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; DEAH-box helicase 9; DExH-box helicase 9; leukophysin; LKP; NDH II; NDH2; NDHII; nuclear DNA helicase II; RHA; RNA helicase A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.