Bethyl Laboratories
Rabbit anti-DLG5 Antibody Affinity Purified
Primary
Catalog #A302-301A
TargetDLG5
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
DLG5 (discs large homolog 5) is a member of the family of discs large (DLG) homologs, a subset of the membrane-associated guanylate kinase (MAGUK) superfamily. The MAGUK proteins are composed of a catalytically inactive guanylate kinase domain, in addition to PDZ and SH3 domains, and are thought to function as scaffolding molecules at sites of cell-cell contact. DLG5 localizes to the plasma membrane and cytoplasm, and interacts with components of adherens junctions and the cytoskeleton. It is proposed to function in the transmission of extracellular signals to the cytoskeleton and in the maintenance of epithelial cell structure [taken from NCBI GeneID:9231].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 800 and 850
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDLG5
Antigen Species
Human
Gene ID9231
Gene Symbol
DLG5
Gene Name
discs large MAGUK scaffold protein 5
Uniprot IDQ8TDM6
Protein Name
Disks large homolog 5
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DLG5 immobilized on solid support.
The epitope recognized by A302-301A maps to a region between residue 800 and 850 of human discs, large homolog 5 using the numbering given in entry NP_004738.3 (GeneID 9231).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-301A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-301A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-301AOpen
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