Bethyl Laboratories
Rabbit anti-DOT1L IHC Antibody Affinity Purified
Primary
Catalog #IHC-00494
TargetDOT1L
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
DOT1L is a histone methyltransferase that methylates nucleosomal histone H3 on lysine 79. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, a domain shown to be necessary for methyltransferase activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1000 and 1050
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetDOT1L
Antigen Species
Human
Gene ID84444
Gene Symbol
DOT1L
Gene Name
DOT1 like histone lysine methyltransferase
Uniprot IDQ8TEK3
Protein Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Alternate Names
DOT1 • DOT1 like histone H3K79 methyltransferase • DOT1-like histone methyltransferase • DOT1-like protein • DOT1-like, histone H3 methyltransferase • H3-K79-HMTase • histone H3-K79 methyltransferase • histone methyltransferase DOT1L • histone-lysine N-methyltransferase, H3 lysine-79 specific • KMT4 • lysine N-methyltransferase 4
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DOT1L immobilized on solid support.
The epitope recognized by IHC-00494 maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00494Open
Product DatasheetPDF · IHC-00494 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00494 · Lot 1 (current lot)Open
Services
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