Bethyl Laboratories
Rabbit anti-Drosha IHC Antibody Affinity Purified
Primary
Catalog #IHC-00389
TargetDrosha
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
Drosha is one of three human RNase III enzymes (Drosha, Dicer, and Argonaute) that play a diverse role in the processing and maturation of RNA. Drosha, along with DGCR8 (DeGeorge Syndrome Chromomsomal Region 8, also known as Pasha in C. elegans and D. melanogaster) is a component of the Microprocessor complex. In this complex, Drosha functions as a double-stranded endoribonuclease critical to the first step in the processing of human microRNAs. During this step, Drosha is responsible for cleaving pri-miRNA to yield hairpin-shaped pre-miRNA for futher processing by the cytoplasmic RNase III enzyme, Dicer.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1075 and 1125
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetDrosha
Antigen Species
Human
Gene ID29102
Gene Symbol
DROSHA
Gene Name
drosha ribonuclease III
Uniprot IDQ9NRR4
Protein Name
Ribonuclease 3
Alternate Names
drosha, double-stranded RNA-specific endoribonuclease • ETOHI2 • HSA242976 • nuclear RNase III Drosha • p241 • Protein Drosha • putative protein p241 which interacts with transcription factor Sp1 • putative ribonuclease III • RANSE3L • ribonuclease 3 • Ribonuclease III • ribonuclease type III, nuclear • RN3 • RNase III • RNASE3L • RNASEN
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Drosha immobilized on solid support.
The epitope recognized by IHC-00389 maps to a region between residue 1050 and 1100 of human double-stranded RNA-specific endoribonuclease using the numbering given in entry NP_037367.3 (GeneID 29102).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Extended incubation with primary antibody (16-24 hours) at room temperature is recommended.
Likely to work with frozen sections.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00389Open
Product DatasheetPDF · IHC-00389 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00389 · Lot 1 (current lot)Open
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