Bethyl Laboratories
Rabbit anti-DTL/CDT2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00495
TargetDTL/CDT2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
DTL/CDT2 is a component of the CUL4A-DDB1 E3 ubiquitinating complex and plays a role in downregulating CDT1, an essential component of pre-replication complexes (Pre-RC) that ensures the genome is replicated only once per cycle. As part of the CUL4A-DDB1 E3 ligase complex DTL/CDT2 is also involved in the regulation of other proteins involved in the G2/M cell cycle checkpoint.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 680 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetDTL/CDT2
Antigen Species
Human
Gene ID51514
Gene Symbol
DTL
Gene Name
denticleless E3 ubiquitin protein ligase homolog
Uniprot IDQ9NZJ0
Protein Name
Denticleless protein homolog
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DTL/CDT2 immobilized on solid support.
The epitope recognized by IHC-00495 maps to a region between residue 680 and the C-terminus (residue 730) of human Denticleless Homolog (Chromatin Licensing and DNA Replicating Factor 2) using the numbering given in entry NP_057532.1 (GeneID 51514).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00495Open
Product DatasheetPDF · IHC-00495 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00495 · Lot 1 (current lot)Open
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