/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-DUBA Antibody
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Primary Antibodies  

Rabbit anti-DUBA Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

DUBA

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 521 and 571

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Deubiquitinating enzyme A (DUBA) is a negative regulator of the innate immune system. DUBA has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains and acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96G74].

55593

OTUD5

OTU deubiquitinase 5

Q96G74

OTU domain-containing protein 5

Alternate Names

deubiquinating enzyme A; deubiquitinating enzyme A; DUBA; OTU domain containing 5; OTU domain-containing protein 5

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.