Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody [BLR295L]
Product Details
Specifications
Immunogen was a recombinant protein representing the extracellular domain of human E-selectin (residues 22-555, NP_000441.2).
Additional Product Information
E-Selectin, aka CD62E, is a cell-surface glycoprotein with a role in immunoadhesion. It is responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining, acting via interaction with SELPLG/PSGL1. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P16581].
Alternate Names
CD antigen CD62E;CD62 antigen-like family member E;CD62E;ELAM;ELAM1;ELAM-1;endothelial adhesion molecule 1;endothelial leukocyte adhesion molecule 1;ESEL;E-selectin;LECAM2;leukocyte endothelial cell adhesion molecule 2;Leukocyte-endothelial cell adhesion molecule 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.