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Rabbit anti-E1B-AP5 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

E1B-AP5

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 806 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications
Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to E1B-AP5 immobilized on solid support.

The epitope recognized by A300-863A-T maps to a region between residue 800 and the C-terminus (residue 856) of human E1B-55kDa-Associated Protein 5 using the numbering given in entry NP_008971.2 (GeneID 11100).

Antibody was affinity purified using an epitope specific to E1B-AP5 immobilized on solid support.

The epitope recognized by A300-863A maps to a region between residue 800 and the C-terminus (residue 856) of human E1B-55kDa-Associated Protein 5 using the numbering given in entry NP_008971.2 (GeneID 11100).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

E1B-AP5 was identified as a protein that interacts with adenovirus type 5 (Ad5) early 55-kDa oncoprotein. EIB-AP5 is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family that can bind RNA and appears to play a role in mRNA processing and transport.

11100

HNRNPUL1

heterogeneous nuclear ribonucleoprotein U like 1

Q9BUJ2

Heterogeneous nuclear ribonucleoprotein U-like protein 1

Alternate Names

adenovirus early region 1B-associated protein 5; E1B 55kDa associated protein 5; E1B-55 kDa-associated protein 5; E1BAP5; E1B-AP5; heterogeneous nuclear ribonucleoprotein U-like protein 1; HNRPUL1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.