Bethyl Laboratories
Rabbit anti-E2F4 Antibody Affinity Purified
Primary
Catalog #A302-133A
TargetE2F4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
E2F4 is a member of the E2F family of transcription factors. It binds DNA at the E2 recognition site found in the promoter region of a number of genes involved in cell cycle regulation and DNA replication. E2F4 binds to pRb, p107, and p130. Via its interaction with p130, E2F4 plays an important role in maintaining G2 arrest during G2 phase checkpoint activation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetE2F4
Antigen Species
Human
Gene ID1874
Gene Symbol
E2F4
Gene Name
E2F transcription factor 4
Uniprot IDQ16254
Protein Name
Transcription factor E2F4
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to E2F4 immobilized on solid support.
The epitope recognized by A302-133A maps to a region between residue 300 and 350 of human E2F transcription factor 4 using the numbering given in entry NP_001941.2 (GeneID 1874).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-133A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-133A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-133AOpen
Citations / Publications
Services
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