/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-E2F8 Antibody
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Primary Antibodies  

Rabbit anti-E2F8 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

E2F8

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 817 and 867

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

E2F8 along with E2F7, functions as an inhibitor of E2F-dependent transcription. E2F8 binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. E2F8 appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96AV8].

79733

E2F8

E2F transcription factor 8

A0AVK6

Transcription factor E2F8

Alternate Names

E2F family member 8; E2F-8; transcription factor E2F8

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.