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Primary Antibodies  

Rabbit anti-E4BP4 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

E4BP4

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 412 and 462

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

E4 promoter-binding protein 4 (E4BP4) functions as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. E4BP4 activates transcription from the interleukin-3 promoter in T-cells and is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16649].

4783

NFIL3

nuclear factor, interleukin 3 regulated

Q16649

Nuclear factor interleukin-3-regulated protein

Alternate Names

E4 promoter-binding protein 4; E4BP4; IL3BP1; interleukin-3 promoter transcriptional activator; interleukin-3-binding protein 1; NFIL3A; NF-IL3A; nuclear factor interleukin-3-regulated protein; transcriptional activator NF-IL3A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.