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Rabbit anti-E6AP Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
E6AP
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 75 and 125
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse, Human
Antigen Species
Human
Stock Concentration
400 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to E6AP immobilized on solid support.

The epitope recognized by A300-351A-T maps to a region between residue 75 and 125 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337).

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Gene & Protein Information

E6AP is an E3 ubiquitin ligase. Inheritance of a mutant maternal copy of this gene results in Angelman syndrome (AS) which is characterized by severe mental retardation, seizures, ataxia, and EEG abnormalities. E6AP interacts with the type 16 and 18 human papillomavirus E6 oncoprotein which results in the targeting of p53 for degradation via the ubiquitin-proteasome system.

7337

UBE3A

ubiquitin protein ligase E3A

Q05086

Ubiquitin-protein ligase E3A

Alternate Names

ANCR, AS, CTCL tumor antigen se37-2, E6-AP, E6AP ubiquitin-protein ligase, EPVE6AP, HECT-type ubiquitin transferase E3A, HPVE6A, human papilloma virus E6-associated protein, human papillomavirus E6-associated protein, oncogenic protein-associated protein E6-AP, renal carcinoma antigen NY-REN-54, ubiquitin-protein ligase E3A

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Citations (4)