/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-EB1 IHC Antibody
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Primary Antibodies  

Rabbit anti-EB1 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

EB1

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IHC-IF

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 218 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

$121.00 $459.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Guinea Pig,

Horse,

Goat,

Dog,

Pig,

Rabbit,

Bovine,

Monkey,

Rat

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to EB1 immobilized on solid support.

The epitope recognized by IHC-00674 maps to a region between residue 245 and 268 of human End-binding protein 1 using the numbering given in entry NP_036457.1 (GeneID 22919).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to EB1 immobilized on solid support.

The epitope recognized by IHC-00674-T maps to a region between residue 245 and 268 of human End-binding protein 1 using the numbering given in entry NP_036457.1 (GeneID 22919).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

End –binding protein 1 (EB1) is a member of the RP/EB family and was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. EB1 also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that EB1 is involved in the regulation of microtubule structures and chromosome stability. [taken from NCBI GeneID:22919].

22919

MAPRE1

microtubule associated protein RP/EB family member 1

Q15691

Microtubule-associated protein RP/EB family member 1

Alternate Names

adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; EB1; end-binding protein 1; microtubule-associated protein RP/EB family member 1

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.