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Rabbit anti-EB1 IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
EB1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 245 and 268
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Goat, Ferret, White-tufted-ear marmoset, Crab-eating macaque, Panda, Monkey, African elephant, Pig, Naked mole rat, Rabbit, Small-eared galago, Chinese hamster, Guinea pig_10141, Little brown bat, Thirteen-lined ground squirrel, Gray short-tailed opossum, Gorilla, Chimpanzee, Northern white-cheeked gibbon, Dog, Bovine, Orangutan, Horse, Rat
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to EB1 immobilized on solid support.

The epitope recognized by IHC-00674-T maps to a region between residue 245 and 268 of human End-binding protein 1 using the numbering given in entry NP_036457.1 (GeneID 22919).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

End –binding protein 1 (EB1) is a member of the RP/EB family and was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. EB1 also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that EB1 is involved in the regulation of microtubule structures and chromosome stability. [taken from NCBI GeneID:22919].

22919

MAPRE1

microtubule associated protein RP/EB family member

Q15691

Microtubule-associated protein RP/EB family member

Alternate Names

adenomatous polyposis coli-binding protein EB1, APC-binding protein EB1, EB1, end-binding protein 1, microtubule-associated protein RP/EB family member 1

Applications

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.