/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ECS (DYKDDDDK) Tag Antibody
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Primary Antibodies  

Rabbit anti-ECS (DYKDDDDK) Tag Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog # A190-102A

Citations (13)
Target:

ECS (DYKDDDDK) Tag

Applications:

ELISA

,

ICC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

Unconjugated (0.1 mg)

A190-102A
$268.00
Qty:
Bulk Request

Product Details

Specifications
Concentration
1 mg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide


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Production Procedures

Rabbits were immunized with ECS, Enterokinase Cleavage Site (DYKDDDDK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By ELISA, Western blot and Immunofluorescence, this antibody reacts with FLAG™.

Country of Origin

USA

Additional Product Information

The entereokinase cleavage site (ECS) tag is an epitope tag with the sequence DYKDDDDK. Its hydrophobic nature helps to maintain the structural integrity of the protein to which it is appended. Further, its nature as a kinase cleavage site allows for its removal from the recombinant protein if needed.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.