/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ECS (DYKDDDDK) Tag Recombinant Monoclonal Antibody [BLRE00G]
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Primary Antibodies  

Rabbit anti-ECS (DYKDDDDK) Tag Recombinant Monoclonal Antibody [BLRE00G]

bethyl
Bethyl Laboratories

Catalog # A191-100

Validated
Target:

ECS (DYKDDDDK) Tag

Applications:

ELISA

,

ICC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLRE00G

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

Product Details

Specifications
Concentration
100 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

The entereokinase cleavage site (ECS) tag is an epitope tag with the sequence DYKDDDDK. Its hydrophobic nature helps to maintain the structural integrity of the protein to which it is appended. Further, its nature as a kinase cleavage site allows for its removal from the recombinant protein if needed.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.