Rabbit anti-EDD1 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-539A maps to a region between residue 600 and 650 of human E3 Ubiquitin Protein Ligase, HECT Domain Containing, 1 using the numbering given in entry NP_056986.2 (GeneID 51366).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EDD1 immobilized on solid support.
The epitope recognized by A303-539A-T maps to a region between residue 600 and 650 of human E3 Ubiquitin Protein Ligase, HECT Domain Containing, 1 using the numbering given in entry NP_056986.2 (GeneID 51366).
Additional Product Information
EDD1 (E3 identified by differential display 1) is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis [taken from NCBI Entrez Gene (Gene ID: 51366)].
Alternate Names
DD5; E3 identified by differential display; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; EDD; EDD1; HECT-type E3 ubiquitin transferase UBR5; hHYD; HYD; hyperplastic discs protein homolog; progestin-induced protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.