Bethyl Laboratories
Rabbit anti-EG5/KIF11 Antibody Affinity Purified
Primary
Catalog #A301-076A
TargetEG5/KIF11
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The kinesin superfamily of proteins consists of forty five KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. EG5/KIF11 plays a role in spindle-pole formation and chromosome positioning and separation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1006 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetEG5/KIF11
Antigen Species
Human
Gene ID3832
Gene Symbol
KIF11
Gene Name
kinesin family member 11
Uniprot IDP52732
Protein Name
Kinesin-like protein KIF11
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to EG5/KIF11 immobilized on solid support.
The epitope recognized by A301-075A maps to a region between residue 1006 and the C-terminus (residue 1056) of human kinesin-related motor protein Eg5 (kinesin family member 11) using the numbering given in entry NP_004514.2 (GeneID 3832).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-076A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-076A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-076AOpen
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