Bethyl Laboratories
Rabbit anti-eIF2A Antibody Affinity Purified
Primary
Catalog #A301-948A
TargeteIF2A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
Image 1 of 2
local_shipping
Order within 10 hours and 17 minutes for shipment by Tuesday, November 17
Product Description
Eukaryotic initiation factor 2A (eIF2A) directs binding of the intiator methionyl-tRNA [Met-tRNA(i)] to 40 S ribosomal subunits in a codon dependent manner. It is a 65 kDa protein that was originally believed to be the functional homolog of prokaryotic IF2. eIF2A displays functional qualities similar to eIF2 which exists as complex of 3 subunits (eIF2-alpha, beta, and gamma); however comparison of eIF2A and eIF2 has shown that these two intitiation factors also display distinct functional abilities.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 450 and 500
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargeteIF2A
Antigen Species
Human
Gene ID83939
Gene Symbol
EIF2A
Gene Name
eukaryotic translation initiation factor 2A
Uniprot IDQ9BY44
Protein Name
Eukaryotic translation initiation factor 2A
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF2A immobilized on solid support.
The epitope recognized by A301-948A maps to a region between residue 450 and 500 of human eukaryotic translation initiation factor 2A, 65kDa using the numbering given in entry NP_114414.2 (GeneID 83939).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-948A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-948A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-948AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1