Bethyl Laboratories
Rabbit anti-eIF2A IHC Antibody Affinity Purified
Primary
Catalog #IHC-00579
TargeteIF2A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Eukaryotic initiation factor 2A (eIF2A) directs binding of the intiator methionyl-tRNA [Met-tRNA(i)] to 40 S ribosomal subunits in a codon dependent manner. It is a 65 kDa protein that was originally believed to be the functional homolog of prokaryotic IF2. eIF2A displays functional qualities similar to eIF2 which exists as complex of 3 subunits (eIF2-alpha, beta, and gamma); however comparison of eIF2A and eIF2 has shown that these two intitiation factors also display distinct functional abilities.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 535 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargeteIF2A
Antigen Species
Human
Gene ID83939
Gene Symbol
EIF2A
Gene Name
eukaryotic translation initiation factor 2A
Uniprot IDQ9BY44
Protein Name
Eukaryotic translation initiation factor 2A
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF2A immobilized on solid support.
The epitope recognized by IHC-00579 maps to a region between residue 525 and 575 of human eukaryotic translation initiation factor 2A, 65kDa using the numbering given in entry NP_114414.2 (GeneID 83939).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00579Open
Product DatasheetPDF · IHC-00579 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00579 · Lot 1 (current lot)Open
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