Bethyl Laboratories
Rabbit anti-EIF2AK1 Antibody Affinity Purified
Primary
Catalog #A302-685A
TargetEIF2AK1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1) is also known as Heme-regulated inhibitor (HRI). EIF2AK1 is regulated and inactivated by Hemin. EIF2AK1 Mediates down-regulation of protein synthesis in response to various stress conditions by the phosphorylation of eIF-2alpha at 'Ser-48' and 'Ser-51'.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 525 and 575
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetEIF2AK1
Antigen Species
Human
Gene ID27102
Gene Symbol
EIF2AK1
Gene Name
eukaryotic translation initiation factor 2 alpha kinase 1
Uniprot IDQ9BQI3
Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 1
Alternate Names
eukaryotic translation initiation factor 2-alpha kinase 1 • HCR • heme regulated initiation factor 2 alpha kinase • heme sensitive initiation factor 2a kinase • heme-controlled repressor • heme-regulated eukaryotic initiation factor eIF-2-alpha kinase • heme-regulated inhibitor • heme-regulated repressor • hemin-sensitive initiation factor 2-alpha kinase • HRI
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to EIF2AK1 immobilized on solid support.
The epitope recognized by A302-685A maps to a region between residue 525 and 575 of human eukaryotic translation initiation factor 2-alpha kinase 1 using the numbering given in entry NP_055228.2 (GeneID 27102).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-685A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-685A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-685AOpen
Citations / Publications
Services
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