Bethyl Laboratories
Rabbit anti-eIF3A/eIF3S10 Antibody Affinity Purified
Primary
Catalog #A302-002A
TargeteIF3A/eIF3S10
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
Image 1 of 2
local_shipping
Order within 20 hours and 45 minutes for shipment by Tuesday, November 3
Product Description
Eukaryotic initiation factor 3 subunit A (eIF3A) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3A is also known as EIF3S10 (eukaryotic initiation factor 3 subunit 7). eIF3A is the largest subunit of the eIF3 complex. eIF3A expression is regulated through the cell cycle. It’s expression peaks in S-phase and it is proposed to play a role in regulating the translation of mRNAs involved in the regulation of cell cycle progression and proliferation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargeteIF3A/eIF3S10
Antigen Species
Human
Gene ID8661
Gene Symbol
EIF3A
Gene Name
eukaryotic translation initiation factor 3 subunit A
Uniprot IDQ14152
Protein Name
Eukaryotic translation initiation factor 3 subunit A
Alternate Names
centrosomin homolog • cytoplasmic protein p167 • EIF3 • eIF3 p167 • eIF3 p180 • eIF3 p185 • EIF3, p180 subunit • eIF3a • eIF3-p170 • EIF3S10 • eIF-3-theta • eIF3-theta • eukaryotic translation initiation factor 3 subunit 10 • eukaryotic translation initiation factor 3 subunit A • eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD) • eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD) • eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa • eukaryotic translation initiation factor 3, subunit 10, 170kD • P167 • p180 • p185 • TIF32
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF3A/eIF3S10 immobilized on solid support.
The epitope recognized by A302-002A maps to a region between residue 1 and 50 of human translation initiation factor 3 subunit A (eukaryotic translation initiation factor 3, subunit 10) using the numbering given in entry NP_003741.1 (GeneID 8661).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-002A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-002A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-002AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1