Bethyl Laboratories
Rabbit anti-eIF3B/EIF3S9 Antibody Affinity Purified
Primary
Catalog #A301-760A
TargeteIF3B/EIF3S9
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
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Product Description
Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargeteIF3B/EIF3S9
Antigen Species
Human
Gene ID8662
Gene Symbol
EIF3B
Gene Name
eukaryotic translation initiation factor 3 subunit B
Uniprot IDP55884
Protein Name
Eukaryotic translation initiation factor 3 subunit B
Alternate Names
eIF3 p110 • eIF3 p116 • eIF3b • eIF-3-eta • EIF3-ETA • EIF3-P110 • EIF3-P116 • EIF3S9 • eukaryotic translation initiation factor 3 subunit 9 • eukaryotic translation initiation factor 3 subunit B • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa • hPrt1 • PRT1 • prt1 homolog
Product Information
Applications
WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF3B/EIF3S9 immobilized on solid support.
The epitope recognized by A301-760A maps to a region between residue 1 and 50 of human eukaryotic translation initiation factor 3, subunit B (eukaryotic translation initiation factor 3, subunit 9) using the numbering given in entry NP_003742.2 (GeneID 8662).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-760A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-760A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-760AOpen
Citations / Publications
Services
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