Bethyl Laboratories
Rabbit anti-eIF3D/EIF3S7 Antibody Affinity Purified
Primary
Catalog #A301-759A
TargeteIF3D/EIF3S7
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
Eukaryotic initiation factor 3 subunit D (eIF3D) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargeteIF3D/EIF3S7
Antigen Species
Human
Gene ID8664
Gene Symbol
EIF3D
Gene Name
eukaryotic translation initiation factor 3 subunit D
Uniprot IDO15371
Protein Name
Eukaryotic translation initiation factor 3 subunit D
Alternate Names
Product Information
Applications
WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF3D/EIF3S7 immobilized on solid support.
The epitope recognized by A301-759A maps to a region between residue 200 and 250 of human eukaryotic translation initiation factor 3, subunit D (eukaryotic translation initiation factor 3, subunit 7) using the numbering given in entry NP_003744.1 (GeneID 8664).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-759A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-759A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-759AOpen
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