Bethyl Laboratories
Rabbit anti-eIF4AIII/EIF4A3 Antibody Affinity Purified
Primary
Catalog #A302-980A
TargeteIF4AIII/EIF4A3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
Eukaryotic initiation factor 4A-III is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. eIF4AIII is an ATP-dependent helicase and a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargeteIF4AIII/EIF4A3
Antigen Species
Human
Gene ID9775
Gene Symbol
EIF4A3
Gene Name
eukaryotic translation initiation factor 4A3
Uniprot IDP38919
Protein Name
Eukaryotic initiation factor 4A-III
Alternate Names
ATP-dependent RNA helicase DDX48 • ATP-dependent RNA helicase eIF4A-3 • DDX48 • DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 • DEAD box protein 48 • eIF4AIII • eIF-4A-III • eIF4A-III • eukaryotic initiation factor 4A-III • eukaryotic initiation factor 4A-like NUK-34 • eukaryotic translation initiation factor 4A • Eukaryotic translation initiation factor 4A isoform 3 • Fal1 • hNMP 265 • MUK34 • NMP 265 • NMP265 • nuclear matrix protein 265 • NUK34 • RCPS
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF4AIII/EIF4A3 immobilized on solid support.
The epitope recognized by A302-980A maps to a region between residue 1 and 50 of human Eukaryotic Translation Initiation Factor 4A3 using the numbering given in entry NP_055555.1 (GeneID 9775).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-980A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-980A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-980AOpen
Citations / Publications
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