Bethyl Laboratories
Rabbit anti-eIF4AIII/EIF4A3 Antibody Affinity Purified
Primary
Catalog #A302-981A
TargeteIF4AIII/EIF4A3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Eukaryotic initiation factor 4A-III is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. eIF4AIII is an ATP-dependent helicase and a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargeteIF4AIII/EIF4A3
Antigen Species
Human
Gene ID9775
Gene Symbol
EIF4A3
Gene Name
eukaryotic translation initiation factor 4A3
Uniprot IDP38919
Protein Name
Eukaryotic initiation factor 4A-III
Alternate Names
ATP-dependent RNA helicase DDX48 • ATP-dependent RNA helicase eIF4A-3 • DDX48 • DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 • DEAD box protein 48 • eIF4AIII • eIF-4A-III • eIF4A-III • eukaryotic initiation factor 4A-III • eukaryotic initiation factor 4A-like NUK-34 • eukaryotic translation initiation factor 4A • Eukaryotic translation initiation factor 4A isoform 3 • Fal1 • hNMP 265 • MUK34 • NMP 265 • NMP265 • nuclear matrix protein 265 • NUK34 • RCPS
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF4AIII/EIF4A3 immobilized on solid support.
The epitope recognized by A302-981A maps to a region between residue 125 and 175 of human Eukaryotic Translation Initiation Factor 4A3 using the numbering given in entry NP_055555.1 (GeneID 9775).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-981A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-981A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-981AOpen
Citations / Publications
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