Rabbit anti-eIF4E Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
eIF4E is a component of the EIF4F initiation complex (eIF4A, eIF4E, eIF4G) that is involved in the processes of mRNA cap recognition, poly (A) binding, and the recruitment of mRNA to the ribosome during protein synthesis. eIF4E plays a role in binding the 5’cap of the mRNA for translation initiation.
Alternate Names
AUTS19; CBP; eIF4E; eIF-4E; EIF4E1; EIF4EL1; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.