Bethyl Laboratories
Rabbit anti-eIF4G3/eIF4GII Antibody Affinity Purified
Primary
Catalog #A301-770A
TargeteIF4G3/eIF4GII
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 23 hours and 28 minutes for shipment by Tuesday, November 17
Product Description
Eukaryotic initiation factor 4 gamma 3 (eIF4G3) is one of 2 eIF4G proteins found in human (eIF4G1 and eIF4G3). eIF4G is a subunit of the eIF4F heterotrimeric complex composed of eIF4A, eIF4E, and eIF4G. eIF4F plays a role in recruiting the ribosome to the 5’-end of capped mRNAs during protein synthesis. In the eIF4F complex, eIF4G functions as a scaffold to bridge the cap-binding subunit, eIF4E, with the RNA helicase, eIF4A.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 850 and 900
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargeteIF4G3/eIF4GII
Antigen Species
Human
Gene ID8672
Gene Symbol
EIF4G3
Gene Name
eukaryotic translation initiation factor 4 gamma 3
Uniprot IDO43432
Protein Name
Eukaryotic translation initiation factor 4 gamma 3
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF4G3/eIF4GII immobilized on solid support.
The epitope recognized by A301-770A maps to a region between residue 850 and 900 of human eukaryotic translation initiation factor 4 gamma, 3 (eukaryotic translation initiation factor 4 gamma II) using the numbering given in entry NP_003751.2 (GeneID 8672).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-770A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-770A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-770AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1