Bethyl Laboratories
Rabbit anti-ELL Antibody Affinity Purified
Primary
Catalog #A301-644A
TargetELL
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 21 hours and 44 minutes for shipment by Tuesday, November 17
Product Description
Eleven-nineteen lysine-rich leukemia protein (ELL) was originally identified in a screen for genes that fuse to MLL (myeloid-lymphoid leukemia) in the t(11;19)(q23;p13.1) breakpoint. ELL is an elongation factor that increases the catalytic rate of RNA pol II by suppressing transient pausing of RNA pol II. ELL has been shown to be essential to development and important to cell growth and survival.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetELL
Antigen Species
Human
Gene ID8178
Gene Symbol
ELL
Gene Name
elongation factor for RNA polymerase II
Uniprot IDP55199
Protein Name
RNA polymerase II elongation factor ELL
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ELL immobilized on solid support.
The epitope recognized by A301-644A maps to a region between residue 150 and 200 of human eleven-nineteen lysine-rich leukemia using the numbering given in entry NP_006523.1 (GeneID 8178).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-644A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-644A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-644AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1