Bethyl Laboratories
Rabbit anti-ENH/PDLIM5 Antibody Affinity Purified
Primary
Catalog #A301-704A
TargetENH/PDLIM5
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The PDZ and LIM domain 5 protein (PDLIM5) bears a PDZ (post-synaptic density-95/discs large/zone occludens-1) domain and three LIM (Lin-11 Isl-1 mec03) zinc-binding domains. PDLIM5 may function as an adaptor protein that mediates protein-protein interactions and interactions with the actin cytoskeleton. PDLIM5 has been implicated in the development of schizophrenia and bipolar disorder.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 546 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetENH/PDLIM5
Antigen Species
Human
Gene ID10611
Gene Symbol
PDLIM5
Gene Name
PDZ and LIM domain 5
Uniprot IDQ96HC4
Protein Name
PDZ and LIM domain protein 5
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ENH/PDLIM5 immobilized on solid support.
The epitope recognized by A301-704A maps to a region between residue 546 and 596 of human enigma homolog (PDZ and LIM domain 5 ) using the numbering given in entry NP_006448.2 (GeneID 10611).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:2000
Documents
Product DatasheetPDF · A301-704A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-704A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-704AOpen
Citations / Publications
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