Bethyl Laboratories
Rabbit anti-EOMES Recombinant Monoclonal Antibody [BLR104H]
Monoclonal Antibodies
Catalog #A700-104
TargetEOMES
HostRabbit
CloneBLR104H
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Order within 20 hours and 40 minutes for shipment by Tuesday, November 17
Product Description
EOMES is a transcription factor in the T-Box family which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. EOMES is also necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. [taken from NCBI Entrez Gene (Gene ID: 8320)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR104H
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetEOMES
Antigen Species
Human
Gene ID8320
Gene Symbol
EOMES
Gene Name
eomesodermin
Uniprot IDO95936
Protein Name
Eomesodermin homolog
Alternate Names
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 125 and 175 of human eomesodermin homolog (EOMES) using the numbering given in entry NP_001265111.1 (Gene ID 8320).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-104 · Lot 2 (current lot)Open
Product DatasheetPDF · A700-104 · Lot 2 (current lot)Open
Safety Data SheetPDF · A700-104Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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